解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The aim of this study was to develop a nondestructive method to quantitate relative amounts of n-3 and n-6 polyunsaturated fatty acid (PUFA) species in vegetable oils and oil seeds using Fourier transform IR spectroscopy (FTIR). The alkene Cbond;H stretching vibrations of unsaturated fatty acids in oils showed IR abso...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10505
更新日期:2003-12-01 00:00:00
abstract::Small-angle x-ray scattering was used for studying intermediate species, isolated after heat-induced aggregation of the A variant of bovine beta-lactoglobulin. The intermediates were separated in two fractions, the heated metastable dimer and heated metastable oligomers larger than the dimer. The pair distance distrib...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10497
更新日期:2003-10-01 00:00:00
abstract::The elucidation of structure-function relationships in insoluble elastin is often approached using elastin-like polypeptides. In this manner, the characterization of the different regions in this extensive biopolymer may be facilitated in a "piece-wise" manner. Our solid-state NMR experiments indicate that (LGGVG)n ha...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10470
更新日期:2003-10-01 00:00:00
abstract::Functional RNAs such as ribosomal RNA and structured domains of mRNA are targets for small molecule ligands that can act as modulators of the RNA biological activity. Natural ligands for RNA display a bewildering structural and chemical complexity that has yet to be matched by synthetic RNA binders. Comparison of natu...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.10410
更新日期:2003-09-01 00:00:00
abstract::Pig citrate synthase (PCS) can be used as a model enzyme to gain some insight into the structural basis of protein thermostability. The thermal unfolding characteristics of the specific secondary structure elements within PCS were monitored in detail by following changes in its amide I band components. The result of o...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10392
更新日期:2003-08-01 00:00:00
abstract::New optical nanoresonance effects enabled us to study the effect of ions on nanometric carbohydrate thin layers on chips. Immobilization was done via spin coating of the derivatized carbohydrate polymer at a metallized chip surface forming ultrathin films (about 50-300 nm thick) followed by photochemical cross-linking...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10371
更新日期:2003-07-01 00:00:00
abstract::The structure of starch was studied using small-angle x-ray scattering (SAXS). The scattering data was modeled by considering a finite stack of alternating lamellae that are allowed to fluctuate both along the layer repeat direction and along the transverse layer direction. Analysis in this way of the SAXS data from s...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10341
更新日期:2003-06-01 00:00:00
abstract::Human islet amyloid polypeptide (hIAPP), or amylin, is a 37 amino acid hormone secreted by pancreatic beta-cells. hIAPP constitutes approximately 90% of the amyloid deposits found in type II diabetic patients. It has been shown that the central region of the peptide (hIAPP(20-29)) constitutes the nucleation site for t...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10305
更新日期:2003-05-01 00:00:00
abstract::Over the past decade a large number of studies have focused attention on the role of nucleosomes as negative and positive regulators of specific nuclear functions. Due to the lack of an analytical method to determine the higher order conformation of the nucleosomal arrays that encompass specific genetic loci (e.g., pr...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10322
更新日期:2003-04-01 00:00:00
abstract::Computer simulations can provide in principle quantitative correlation between the structures of DNA polymerases and the replication fidelity. This paper describes our progress in this direction. Using several theoretical approaches, including the free energy perturbation (FEP), linear response approximation (LRA), an...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10244
更新日期:2003-03-01 00:00:00
abstract::Spontaneous cortical activity of single neurons is often either dismissed as noise, or is regarded as carrying no functional significance and hence is ignored. Our findings suggest that such concepts should be revised. We explored the coherent population activity of neuronal assemblies in primary sensory area in the a...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10273
更新日期:2003-03-01 00:00:00
abstract::Seven water-insoluble (1 --> 3)-beta-D-glucan fractions TM8-1 to TM8-7 with weight-average molecular mass M(w) ranged from 2.22 to 77.4 x 10(4) obtained from the sclerotia of Pleurotus tuber-regium were carboxymethylated to produce the water-soluble fractions CTM8-1 to CTM8-7 with M(w) ranged from 3.87 to 87.8 x 10(4)...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10277
更新日期:2003-02-01 00:00:00
abstract::Peptide-bond modification via N-hydroxylation has been explored as a strategy for metal coordination to induce conformational rigidity and orient side chains for specific molecular recognition. N-Hydroxyamides were prepared by reacting N-benzyloxyamino acid esters or amides with Fmoc-AA-Cl/AgCN (Fmoc: 9-fluorenylmetho...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10471
更新日期:2003-01-01 00:00:00
abstract::Gelsolin is an actin-severing protein whose action is initiated by Ca(2+) and inhibited by binding to phosphorylated inositol lipid or phosphoinositides. The regions of gelsolin responsible for phosphoinositide binding are comprised of residues 150-169 (G150-169) and 135-142 (G135-142). The corresponding peptides poss...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10375
更新日期:2003-01-01 00:00:00
abstract::The present study characterizes the unfolding and folding processes of recombinant manganese peroxidase. This enzyme contains five disulfide bonds, two calcium ions, and one heme prosthetic group. Circular dichroism in the far UV was used to monitor global changes of the protein secondary structure, whereas UV-visible...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10283
更新日期:2003-01-01 00:00:00
abstract::Surface-enhanced Raman spectroscopy (SERS) in a silver sol assisted by density functional theory (DFT) calculations is shown to be a promising tool in the characterization of platinum complexes and their interaction with nucleic acid bases. This is demonstrated using cisplatin and guanine as a model. The energies and ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10480
更新日期:2003-01-01 00:00:00
abstract::The formation mechanism of an alanine-based peptide has been studied by all-atom molecular dynamics simulations with a recently developed all-atom point-charge force field and the Generalize Born continuum solvent model at an effective salt concentration of 0.2M. Thirty-two simulations were conducted. Each simulation ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10216
更新日期:2003-01-01 00:00:00
abstract::In this study a novel general approach is presented that allows for a straightforward design of receptor binding assays. This principle of a receptor binding assay is applied to the estrogen receptor, which is important in the management of breast cancer and for the estimation of the estrogenic potency of chemicals in...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10402
更新日期:2003-01-01 00:00:00
abstract::A series of novel nonpeptide inhibitors of the pp60(c-Src) (Src) SH2 domain is described that exploit multifunctional group replacement of the phenylphosphate moiety of phosphotyrosine (pTyr). Relative to an x-ray structure of citrate complexed to the pTyr binding site of the Src SH2 domain, these nonpeptide ligands i...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10600
更新日期:2003-01-01 00:00:00
abstract::The applicability of the FTIR attenuated total reflectance technique for in situ monitoring of plant physiological processes such as leaf senescence and aging has been examined. Difference spectra obtained by subtracting the spectrum of the young plant leaf from that of the older one revealed positive bands at 1650-15...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10297
更新日期:2003-01-01 00:00:00
abstract::Fourier transform IR (FTIR) spectroscopy has been successfully applied in recent years to examine the functional and structural properties of the membrane protein rhodopsin, a prototype G protein coupled receptor. Unlike UV-visible spectroscopy, FTIR spectroscopy is structurally sensitive. It may give us both global i...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.10407
更新日期:2003-01-01 00:00:00
abstract::Molecular dynamics (MD) simulations on stem loop 2 of U1 small nuclear RNA and a construct of the U1A protein were carried out to obtain predictions of the structures for the unbound forms in solution and to elucidate dynamical aspects of induced fit upon binding. A crystal structure of the complex between the U1A pro...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10251
更新日期:2002-12-15 00:00:00
abstract::A series of iota-carrageenans containing different amounts of nu-carrageenan (0-23 monomer %) have been prepared from neutrally extracted carrageenan of Eucheuma denticulatum. nu-Carrageenan is the biochemical precursor of iota-carrageenan. The conformational order-disorder transition and rheological properties of the...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10250
更新日期:2002-11-15 00:00:00
abstract::Three new polyoxaalkyl esters of lasalocid are synthesized. Their ability to form complexes with Li(+) and Na(+) cations is studied using multinuclear NMR methods, FTIR spectroscopy in the middle and far IR regions, and mass spectrometry. It is found that lasalocid esters form only 1:1 complexes with the metal cations...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10194
更新日期:2002-10-15 00:00:00
abstract::The methods used to study the relative reaction rates of 45 different aliphatic and aromatic carboxylic acids when coupled to resin-bound amino acid amides is described. Competition experiments involving the coupling of incoming carboxylic acids to resin-bound amino acid amides were performed. The relative composition...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10206
更新日期:2002-10-05 00:00:00
abstract::In this article, the pulsed double electron-electron resonance in electron spin-echo (PELDOR) technique is applied to study the self-aggregation of spin-labeled zervamicin IIA, a hexadecapeptide antibiotic of fungal origin, which is known to form ion channels in a phospholipid double layer. Measurements of the ion cha...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10208
更新日期:2002-09-01 00:00:00
abstract::Interactions between the diketopiperazine cyclo-alanylglycine and four fluorinated alcohols in water-fluoroalcohol mixtures were examined by (1)H[(19)F] intermolecular nuclear Overhauser effects (NOE) experiments. The alcohols studied were trifluoroethanol, hexafluoroacetone trihydrate, 1,1,1,3,3,3-hexafluoroisopropan...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10176
更新日期:2002-08-15 00:00:00
abstract::A model of protein-chaperone interaction as a two-phase (unfolding/refolding) iterative annealing mechanism able to promote structural segregation of hydrophobic and hydrophilic monomers and thereby facilitate access to nativelike states has recently been applied successfully to two 22-mers of the Honeycutt and Thirum...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10148
更新日期:2002-07-15 00:00:00
abstract::We present a method for analysis of a fitness landscape of a biopolymer with significantly epistatic sites. The analysis is based on a quasi-additive fitness model. The fitness model is constructed with additive terms conducted by "site-fitness" and epistatic terms conducted by "pair-fitness," where the site-fitness i...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10126
更新日期:2002-07-05 00:00:00
abstract::Partially acetylated, high molecular weight glucuronans were produced by a Sinorhizobium meliloti mutant strain. Two native glucuronan samples with various degrees of acetylation were sonicated to obtain lower molecular weight samples and with low viscosity suitable for chemical modification and (13)C NMR experiments....
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10134
更新日期:2002-06-01 00:00:00
abstract::The conformational study on Arg-Gly-Asp (RGD)-containing tetrapeptides in the unhydrated and hydrated states has been carried out using the force field ECEPP/3 and the hydration shell model. The tetrapeptides studied here are H-RGDX-OH (X = Trp, Tyr, Phe, Leu, Val, Cys, Gln, and Ser), which show the inhibitory activit...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10067
更新日期:2002-04-15 00:00:00
abstract::The general properties of hydroxyethylcellulose (HEC) grafted with 3-glycidoxypropyltrimethoxysilane (GPTMS) or 3-glycidoxypropylmethyldiethoxysilane (GPDMS) were studied for potential biomedical applications. The graft involved a Williamson reaction between the free hydroxyl function of HEC and the epoxy function of ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10053
更新日期:2002-04-05 00:00:00
abstract::13C and (31)P NMR spectra of a transmembrane peptide, [1-(13)C]Ala(14)-labeled A(6-34), of bacteriorhodopsin incorporated into dimyristoylphosphatidylcholine (DMPC) bilayer were recorded to clarify its dynamics and orientation in the lipid bilayer. This peptide is shown to take an alpha-helical form both in liquid cry...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10021
更新日期:2002-02-01 00:00:00
abstract::The coupling of nuC-O and deltaO-D vibrations in the 1200-900 cm(-1) IR range leads to band shifting in opposite directions, which provides information on intramolecular hydrogen bonding of carbohydrates in aqueous solution. The aqueous solution IR spectra of 2-acetamide-1,6-anhydro-2-deoxy-D-glucopyranose and cis-1,2...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10024
更新日期:2002-01-01 00:00:00
abstract::The aim of this study was to investigate the effect of dopa and catecholamines on the generation of oxygen active species during oxidation of farmorubicin by "Fenton-like" reagents using chemiluminescent and spectrophotometric techniques. The tested catechols were found to reduce the light emission accompanying oxidat...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10161
更新日期:2002-01-01 00:00:00
abstract::Side-chain lactam bridges linking amino acid residues that are spaced several residues apart in the linear sequence offer a convenient and flexible method for introducing conformational constraints into a peptide structure. The availability of a variety of selectively cleavable protecting groups for amines and carboxy...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.10203
更新日期:2002-01-01 00:00:00
abstract::Fluorescence binding measurements and molecular modeling were employed to study the interaction of hypericin (Hyp) with human (HSA), rat (RSA), and bovine (BSA) serum albumins. Fluorescence emission data show the solubility of Hyp increasing in the order BSA, HSA, and RSA. Molecular modeling was used to construct the ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10110
更新日期:2002-01-01 00:00:00
abstract::Vibrational spectroscopy coupled with thermogravimetry (TG) and differential scanning calorimetry (DSC) was used to characterize racemic propanoic acid, 2-methyl-5,6,7,8-tetrahydro-6-(methylamino)-1,2-naphthalenediyl ester hydrochloride (CHF-1035), which is a new DA2 dopaminergic receptor/alpha2 agonist and beta block...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10089
更新日期:2002-01-01 00:00:00
abstract::Cytochrome c3 from Desulfovibrio gigas is electrostatically adsorbed on Ag electrodes coated with self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid. The redox equilibria and electron transfer dynamics of the adsorbed four-heme protein are studied by surface enhanced resonance Raman spectroscopy. Immobiliz...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10101
更新日期:2002-01-01 00:00:00
abstract::Inhibitor design against viral targets must take into account the peculiar characteristics of viral biology-in particular, the plasticity of their replicative machinery. This includes maturational cleavage of the polyprotein, which is mediated by virally encoded proteases. Designing against a movable target is particu...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.10230
更新日期:2002-01-01 00:00:00